Proteomics/ Metabolomics/ Mass spectrometry
Please find below an overview of equipment that falls under Proteomics, Metabolomics and Mass spectrometry. Scroll down to find more information per equipment (e.g. contact and registration details).
| Equipment | Status |
|---|---|
| Akta Pure System | In use |
| Seahorse XFe96 Analyzer | In use |
| UPLC/ MS-MS (Xevo TQ-QS) | In use |
| MassArray (Agena) | In negotiation |
Akta Pure System
Status: in use
Location: Ee-762
Registration: paper calendar
Contact person:
Joyce Lebbink
E: j.lebbink@erasmusmc.nl
The Akta Pure from GE Healthcare is equipment for purification of proteins. The set-up contains multiple sensors and valves to allow for rapid and efficient throughput and optimisations of runs. The system consist of multiple pumps and valves to flow different buffers over multiple chromatography columns, on which protein mixtures in cell lysates are separated according to size, charge or affinity for certain resins. Sensors monitor UV absorption at any wavelength, pH and conductivity of the buffers, pressure and air in the system. A fraction collector allows collection of the desired protein fractions. The system is controlled via a user-friendly software package and is operated at 4 degrees.
Seahorse XFe96 Analyzer
Status: in use
Location: Be-431
Registration: please contact Cor Berrevoets
Contact person:
Cor Berrevoets
E: c.berrevoets@erasmusmc.nl
The Agilent Seahorse XFe96 Analyzer (S7800B) is designed to facilitate studies into cellular metabolism. To this end, the Analyzer measures and computes the oxygen consumption rate (OCR) and the extracellular acidification rate (ECAR) of live cells in a 96-well plate format. OCR and ECAR rates are key indicators of mitochondrial respiration, glycolysis and ATP production. Together these measurements provide a systems-level view of cellular metabolic function in cultured tumor cells, immune cells as well as ex vivo patient samples.
UPLC/ MS-MS (Xevo TQ-QS)
Status: in use
Location: Be-461
Registration: no open registration. Please contact Peter de Bruijn.
Contact person:
Peter de Bruijn
E: p.debruijn@erasmusmc.nl
In 2020 we took delivery of a new Waters TQ-XS triple quadrupole mass spectrometer, linked to an ACQUITY UPLC system with the possibility of 2D LC technology. The Laboratory of Translational Pharmacology is the first laboratory within the Erasmus MC to have this mass spectrometer. This device offers the possibility to measure very low concentrations of drug levels in body fluids of patients. In addition, we hope in the future to be able to develop methods for measuring drug concentrations in tumor material. These analysis will enable the laboratory to better map the distribution of anti-cancer agents in the body and to explore to what extent anti-cancer agents are able to reach the tumor.
MassArray (Agena)
Status: in negotiation
Location: not yet known
Registration: not yet known
Contact person:
Maurice Jansen
E: m.p.h.m.jansen@erasmusmc.nl
This system is mass spectrometer to accurately, rapidly and cost-effectively measures highly multiplexed PCR-derived amplicons.